IMR Press / FBE / Volume 3 / Issue 3 / DOI: 10.2741/E306

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

1H NMR-based metabolomic analysis of urine from preterm and term neonates

Show Less
1 Department of Toxicology, University of Cagliari, Italy
2 Department of Pediatrics and Clinical Medicine - Section of Neonatal Intensive Care Unit, University of Cagliari, Italy
3 Department of Neurological Sciences, University of Cagliari, Italy
4 Department of Chemical Sciences, University of Cagliari, Italy
5 Pediatric Division, Pescia Hospital, Italy
6 Department of Biological Sciences, University of Calgary, Canada
7 Department of Maternal, Fetal and Neonatal Medicine, C. Arrigo Children’s Hospital, Alessandria, Italy

*Author to whom correspondence should be addressed.

Academic Editor: Diego Gazzolo

Front. Biosci. (Elite Ed) 2011, 3(3), 1005–1012; https://doi.org/10.2741/E306
Published: 1 June 2011
(This article belongs to the Special Issue Clinical and biochemical markers and fetal-neonatal development)
Abstract

Metabolomics is a technique used to non-invasively determine metabolic status of an organism. Aim of our study was to analyze urinary metabolic profiles in term and preterm infants in order to identify gestational age-related metabolic differences and to predict metabolic maturity at birth. Twenty-six healthy term infants and 41 preterm infants were prospectively enrolled. A urine sample was collected non-invasively within the first hours of life. Samples were analyzed by proton nuclear magnetic resonance (1H NMR) spectroscopy and NMR urine spectra were analyzed by multivariate statistical analysis. Distinct metabolic patterns were found between term infants and preterm infants, as well as between preterm infants of 23-32 weeks' gestation and those of 33-36 weeks' gestation. Individual metabolites discriminating between these groups were hippurate, tryptophan, phenylalanine, malate, tyrosine, hydroxybutyrate, N-acetyl-glutamate, and proline. Metabolomic analysis revealed distinct urinary metabolic profiles in neonates of different gestational ages, and identified the discriminating metabolites. This holistic approach appears to be a promising tool for investigating newborn metabolic maturation over time, and might lead to a tailored management of neonatal disorders.

Keywords
Metabolomics
Preterm Neonates
Non Invasive Analysis
Share
Back to top