IMR Press / FBE / Volume 3 / Issue 3 / DOI: 10.2741/E293

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Use of hr3 enhancer and P74 TM domain in baculovirus surface display

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1 The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education and The State Key Laboratory of Bio-membrane and Membrane Bio-engineering, College of Life Sciences, Peking University, Beijing 100871, P. R. China
2 The Center for Theoretical Biology, Peking University, Beijing 100871, P. R. Chin
3 Institute of Virology, Zhejiang Provincial Center For Disease Prevention and Control, Hangzhou, Zhejiang 310009, P. R. China
4 Neuroscience Research Institute and Infectious Disease Center, Health Science Center, Peking University, Beijing 100191, P.R.China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2011, 3(3), 856–863; https://doi.org/10.2741/E293
Published: 1 January 2011
Abstract

Baculovirus surface display technique provides a new platform for novel vaccine research and production. Unfortunately, the low display efficiency in current methods and the waste of occlusion-derived virion (ODV) products limited its application. We investigated the use of two motifs, BmNPV hr3 and transmembrane domain of P74 (P74TM), in display. Budding virus (BV) with hr3 showed a 14.2-time enhanced display efficiency than the current recombinant BV. Hemagglutinin (HA) protein of H5N1 influenza virus was displayed by using 3 different vectors to ensure the improvement of display efficiency and the characters of displayed protein in recombinant baculovirus. Immunoassay demonstrated that the recombinant BV with TM/CTD of vsvG protein, and hr3 could induce the highest level of neutralizing antibody against HA, suggesting that the optimized HA displaying BV could be a novel live virus-based vaccine candidate for influenza virus. In addition, GFP fused with the P74TM could be anchored to the ring zone in infected cell and the ODV envelope, which may luminate a new direction for ODV display and provide a promising strategy to use ODV products.

Keywords
Baculovirus Surface Display
hr3
P74
GP64
H5N1
Transmembrane Domain
BV
ODV
Hemagglutinin
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