IMR Press / FBE / Volume 3 / Issue 3 / DOI: 10.2741/E289

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with Frontiers in Bioscience.

Effect of two pasteurization methods on the protein content of human milk
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1 CNR – Institute of the Science of Food Production, Grugliasco, Turin, Italy
2 Italian Association of Human Milk Banks, Milan, Italy
3 Neonatal Intensive Care Unit, Department of Pediatric and Adolescence Science, University of Turin, Turin, Italy
4 Bufalini Hospital, Cesena, Italy
5 Department of Neonatology, Macedonio Melloni Maternity Hospital, Milan, Italy

*Author to whom correspondence should be addressed.


Front. Biosci. (Elite Ed) 2011, 3(3), 818–829;
Published: 1 June 2011

The Holder method is the recommended pasteurization method for human milk banks, as it ensures the microbiological safety of human milk (HM). The loss of some biologically active milk components, due to the heat treatment, is a main limit to the diffusion of donor HM. High-temperature short-time (HTST) pasteurization may be an alternative to maintain the nutritional and immunological quality of HM. The aim of the present study was to compare the impact of Holder and HTST pasteurization on the HM protein profile. The protein patterns of HTST-treated milk and raw milk were similar. The Holder method modified bile salt-stimulated lipase, lactoferrin and components of the immune system. The HTST method preserved the integrity of bile salt-stimulated lipase, lactoferrin and, to some extent, of IgAs. Holder pasteurization decreased the amount of bile salt-stimulated lipase and inactivated the remaining molecules, while the HTST method did not alter its activity. Pasteurization increased the bioavailable lysine quantity. HTST pasteurization seems to better retain the protein profile and some of the key active components of donor HM.

Donor Milk
Very Low Weight Infants
Human Milk Banks
Proteins Profile
Mass Spectrometry
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