IMR Press / FBE / Volume 3 / Issue 2 / DOI: 10.2741/E272

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
The role of nuclear factor-kappa-B p50 subunit in the development of endometriosis
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1 Shanghai OB/GYN Hospital, Fudan University, Shanghai, China 200011
2 Dept. of Obstetrics and Gynecology, Cixi People’s Hospital, Cixi, Zhejiang, China 315300
3 Vascular Biology Center and Division of Hematology, Oncology and Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, MN 55455, USA

*Author to whom correspondence should be addressed,

Academic Editor: Khaleque Newaz Khan

Front. Biosci. (Elite Ed) 2011, 3(2), 591–603; https://doi.org/10.2741/E272
Published: 1 January 2011
(This article belongs to the Special Issue Frontiers in endometriosis)
Abstract

p50 is a member of the NF-kappaB family known to be involved in endometriosis. To gain insight into the roles of p50 in the development of endometriosis, we cross-transplanted endometrial fragments from p50 knockout mice to wild-type mice and vice versa, and also autotransplanted the fragments within the knockout and wild-type mice, inducing endometriosis. We then evaluated the size of the endometrial implants, and immunoreactivity to phosphorylated p65 (p-p65), PKCepsilon and TRPV1 in ectopic and eutopic endometrium as well as in vagina. We found that p50 deletion significantly reduces the size of endometrial implants. The immunoreactivity to p-p65 and PKCepsilon, but not TRPV1, was reduced in endometrial implants in p50 knockout mice. Deletion of p50 significantly reduced p-p65 and PKCepsilon, but not TRPV1, expression in eutopic endometrium and vagina. It also disrupts NF-kappaB activation and PKCepsilon expression in eutopic and vagina, suggesting the role of NF-kappaB in regulating PKCepsilon, which plays an important role in nociception. These data show that p50 is involved in the development of endometriosis and may be a promising therapeutic target.

Keywords
Endometriosis
inflammation
NF- kappaB
p50
p65
PKCepsilon
TRPV1
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