IMR Press / FBE / Volume 3 / Issue 2 / DOI: 10.2741/E256

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Sage components enhance cell death through nuclear factor kappa-B signaling
Show Less
1 Department of Biology, American University of Beirut, Beirut, Lebanon
2 Division of Clinical Pharmacology, Department of Medical Sciences, University Hospital, SE-751 85 Uppsala, Sweden

*Author to whom correspondence should be addressed,

Academic Editor: Hala Gali-Muhtasib

Front. Biosci. (Elite Ed) 2011, 3(2), 410–420; https://doi.org/10.2741/E256
Published: 1 January 2011
(This article belongs to the Special Issue New emerging anticancer lead compounds from plants)
Abstract

The sage components linalyl acetate (Ly) and alpha-terpineol (Te) exhibit synergistic anti-proliferative effects. We investigated the effects of Ly and Te on NF-KB signaling in HCT-116 colon cancer cells. Ly and Te combinations dose-dependently reduced HCT-116 viability at non-cytotoxic concentrations. Combination treatment induced 30%-60% increase in PreG1 through induction of apoptosis and necrosis. DNA binding assays revealed that combination treatment suppressed both basal and TNF-alpha-induced NF-KB activation. This suppression correlated with the inhibition of p65 nuclear translocation and IKB-alpha degradation. The lack of change in IKK expression levels or inhibition in IKB-alpha phosphorylation suggest the involvement of an IKK-independent mechanism. Ly and Te combination was found to downregulate the expression of NF-KB-regulated antiapoptotic and proliferative gene products. Separate treatments and drug combinations significantly decreased DNA binding activity of NF-KB which led to the potentiation of cell death induced by the colon cancer drugs oxaliplatin and 5-FU. These results indicate that Ly and Te anticancer activities are partly mediated through the suppression of NF-KB activation, suggesting their use in combination with chemotherapeutic agents to induce apoptosis.

Keywords
Colon Cancer
Herbal Medicine
Linalyl Acetate
NF kappab
Alpha-Terpeniol
Sage
Share
Back to top