IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E253

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Hepatitis D antigens cause growth retardation and brood-size reduction in C. elegans
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1 Department of Life Science, Chang Gung University, Tao-Yuan 333, Taiwan
2 Graduate Institute of Biomedical Sciences, Chang Gung University, Tao-Yuan 333, Taiwan
3 National Yang-Ming University, School of Life Science, Institute of Microbiology and Immunology, Taipei 112, Taiwan

*Author to whom correspondence should be addressed.

Academic Editor: Lo Szecheng

Front. Biosci. (Elite Ed) 2011, 3(1), 380–390; https://doi.org/10.2741/E253
Published: 1 January 2011
(This article belongs to the Special Issue Molecular biology and pathogenesis of HBV and HDV)
Abstract

Caenorhabditis elegans is a model organism that has been used to study human bacterial and viral pathogenesis. We report here the expression of human hepatitis delta viral antigens (HDAg) in C. elegans and measure the effect on the sterility, growth, and brood size in transgenic worms. Expression of HDAg under two different promoters, fib-1 (a ubiquitous promoter) and myo-2 (a pharynx-specific promoter), was achieved in C. elegans using dicistronic or tricistronic vectors derived from the operon CEOP5428. Transgenic worms expressing HDAg ubiquitously resulted in 20% to 70% sterility while those expressing HDAg in the pharynx displayed 70% sterility. Most of worms expressing HDAg in pharynx were arrested at larvae stage 2 or 3 and displayed a 70% reduction in brood size. Domain mapping experiments suggested that the nuclear localization signal of HDAg is required for the observed effect. Heat-shock induction of HDAg expression revealed that L4 larvae were the most sensitive to brood size reduction. These studies demonstrate that C. elegans can provide an additional model for studying HDAg interactions with host targets.

Keywords
HDV
delta antigens
C. elegans
Transgenic Animals
Brood Size
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