IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E241

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Isolation and expansion of adipose-derived stem cells for tissue engineering
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1 Department of Health Science and Technology, Aalborg University, Aalborg, Denmark. trinef@hst.aau.dk
2 Department of Pharmacology, Aarhus University, Denmark
3 Orthopaedic Research Laboratory, Aarhus University Hospital, Denmark

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2011, 3(1), 256–263; https://doi.org/10.2741/E241
Published: 1 January 2011
Abstract

For treatment of cardiac failure with bone marrow-derived mesenchymal stem cells, several clinical trials are ongoing. However, more attention is gathering on the use of adipose tissue-derived stem cells (ASCs). This paper describes the optimization of isolation and propagation of ASCs for subsequent clinical use. In the isolation step, several enzymes were compared with respect to yield of nucleated cells and precursor cells. Our results showed, that the interdonor variablility was greater than differences between individual enzymes. For propagation of cells, different types of media, sera and serum replacers were evaluated regarding their ability to support cell growth and preserve differentiation potential. Most of serum replacers proved inferior to fetal calf serum. Among the media tested, modified Eagle's media alpha was superior in promoting cell growth while preserving the ability to differentiate. Also, the effect of cell seeding density and hypoxic culture was evaluated. In this study, we show that it is possible to maximize cell yield regardless of donor individual characteristics by simple manipulations of media composition, cell seeding density and gaseous environment.

Keywords
adipose tissue
mesenchymal stem cells
cell proliferation
cell differentiation
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