IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E240

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Shotgun proteomics analysis on maize chloroplast thylakoid membrane
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1 Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 26 Hexing road, Harbin, 150040, China
2 Department of Pharmacy, Daqing Branch, Harbin Medical University, Daqing 163319, China
3 Library of Northeast Forestry University, 26 Hexing road, Harbin, 150040, China
4 Laboratory of Genetics, Faculty of Biology, Harbin Normal University, 50 Hexing road, Harbin, 150080, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2011, 3(1), 250–255; https://doi.org/10.2741/E240
Published: 1 January 2011
Abstract

In this study we initiated a proteomic investigation of the maize thylakoid membrane by using a shotgun proteomic approach based on LC-MSE. A total of 34 maize thylakoid membrane proteins were identified, the majority of which are primarily involved in photosynthesis, including the light-reaction and carbon assimilation. It is noteworthy that all of the core subunits of the Photosystem II were identified in our search. Proteins involved in other processes, such as iron storage, were also detected in our study. The quantity of each identified protein was also determined. Of interest, we discovered that the amount of the three ATP synthase subunits were not equivalent, suggesting that these proteins perform other functions in addition to ATP synthesis. To our knowledge this is the first extensive proteomic investigation of the maize thylakoid membrane, and will likely enable further study of maize photosynthesis and chloroplast development.

Keywords
Maize
Photosynthesis
Proteome
Shotgun
Thylakoid Membrane
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