IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E235

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Identifying serum biomarkers for TACE therapy efficiency of hepatocellular carcinoma
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1 Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

*Author to whom correspondence should be addressed.

Academic Editor: Xueji Zhang

Front. Biosci. (Elite Ed) 2011, 3(1), 212–220; https://doi.org/10.2741/E235
Published: 1 January 2011
(This article belongs to the Special Issue Biosensors and their applications)
Abstract

Transcatheter Arterial Chemoembolization (TACE) is the first line of treatment in inoperable hepatocellular carcinoma. Magnetic affinity beads can be used to extract peptides from un-fractionated serum samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can detect the presence and the molecular mass of peptides. In this study, we used a highly optimized ClinProt-matrix-assisted laser desorption/ionization time-of flight mass spectrometer (MALDI-TOF-MS) to screen hepatocellular carcinoma markers for TACE. 40 sera from 20 patients, including before and after TACE to explore those biomarkers, might be related with therapy efficiency, and some of the patients who received another therapy were analyzed as well. The spectra were analyzed statistically using FlexAnalysis™ and Clin-Prot™ bioinformatic software. The seven most significant differential peaks (p<0.0.5) were selected out by ClinProTool software to identify hepatocellular carcinoma markers for TACE therapy. Furthermore, the differential peptide of 3883Da was identified as plasma serine protease inhibitor precursor (Protein C inhibitor). This study provides a direct link between peptide marker profiles and TACE therapy, and the markers may have clinical utility for monitoring efficiency of therapy.

Keywords
hepatocellular carcinoma
TACE
MALDITOF-MS
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