IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E232

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Heterogeneity of mitochondrial energy metabolism in classical triphasic Wilms' tumor
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1 Department of Pediatrics, University Hospital Salzburg, Paracelsus Medical University, Muellner Hauptstr. 48, A-5020 Salzburg, Austria
2 Department of Pathology, University Hospital Salzburg, Paracelsus Medical University, Muellner Hauptstr. 48, A-5020 Salzburg, Austria
3 Institute of Human Genetics and Anthropology, Heinrich Heine University of Duesseldorf, D40001 Duesseldorf, Germany
4 Institute of Pathology, Medical University of Graz, A-8036 Graz, Austria

*Author to whom correspondence should be addressed.

Academic Editor: Anna Czarnecka

Front. Biosci. (Elite Ed) 2011, 3(1), 187–193; https://doi.org/10.2741/E232
Published: 1 January 2011
(This article belongs to the Special Issue Frontiers in molecular medicine)
Abstract

Metabolic changes are observed in a variety of tumors. The nature of the changes in aerobic energy metabolism differs between tumor types. Therefore, immunohistochemical staining, enzymatic measurements and immunoblot analysis were used to determine alterations of oxidative phosphorylation (OXPHOS) in classic triphasic Wilms' Tumor (WT). Our studies revealed that the epithelial, stromal and blastemal elements of this tumor differ in their energy metabolism. Compared to unaffected kidney tissue, normal mitochondrial mass was observed in the epithelial and blastemal regions of WT, whereas the stroma showed a massive down-regulation of mitochondria, as indicated by low porin content, low citrate synthase activity, and reduced mtDNA copy number. All OXPHOS enzyme activities were reduced in all WT samples, with the exception of two epithelial-dominant cases, which showed up-regulation of complex III activity compared to control kidney tissues. Interestingly, our studies show that, even within a specific tumor entity, cell-type-specific alterations of aerobic energy metabolism can occur, although all cell types showed a clear tendency toward a reduced aerobic energy metabolism.

Keywords
OXPHOS
Respiratory Chain
Stroma
Blastema
Epithelial Mesenchymal Transition
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