IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E230

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
KHYG-1 and NK-92 represent different subtypes of LFA-1-mediated NK cell adhesiveness
Show Less
1 Blood Services Group, Health Sciences Authority, 11 Outram Road, Singapore,169078
2 School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551
3 Department of Haematology, St. George's Hospital and Medical School, Blackshaw Road, London, SW17 0PE, UK

*Author to whom correspondence should be addressed.

Academic Editor: Garnet Suck

Front. Biosci. (Elite Ed) 2011, 3(1), 166–178; https://doi.org/10.2741/E230
Published: 1 January 2011
(This article belongs to the Special Issue Cellular therapy and vaccine development)
Abstract

Novel cancer cellular therapy approaches involving long-term ex vivo IL-2 stimulated highly cytotoxic natural killer (NK) cells are emerging. However, adhesion properties of such NK cells are not very well understood. Herein, we describe the novel observation of permanently activated alphaLbeta2 integrin leukocyte function-associated antigen (LFA)-1 adhesion receptor in long-term IL-2 activated NK cells and the permanent NK cell lines KHYG-1 and NK-92. We show that such cytokine activated NK effectors constitutively adhered to the LFA-1-ligand ICAM-1, whereas binding to the lower affinity ligand ICAM-3 required additional exogenous activating conditions. The results demonstrate an extended conformation and an intermediate affinity state for the LFA-1 population expressed by the NK cells. Interestingly, adhesion to ICAM-1 or K562 induced pronounced cell spreading in KHYG-1, but not in NK-92, and partially in long-term IL-2 stimulated primary NK cells. It is conceivable that such differential adhesion characteristics may impact motility potential of such NK effectors with relevance to clinical tumor targeting. KHYG-1 could be a useful model in planning future targeted therapeutic approaches involving NK effectors with augmented functions.

Keywords
Adoptive Immunotherapy
cancer
Natural killer (NK) cell
Lymphokine Activated Killer
LAK cells
leukocyte function-associated antigen
LFA-1
Intercellular Adhesion Molecule
ICAM-1
adhesion
cell spreading
NK-92
KHYG-1
Share
Back to top