IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E228

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Aberrant methylation as a main mechanism of TSGs silencing in PTC
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1 Department of Molecular Bases of Medicine, Medical University of Lodz, Pomorska St. 251, 92-213 Lodz, Poland
2 Department of General, Oncological and Endocrine Surgery, Medical University of Lodz, Polish Mother’s Memorial Hospital – Research Institute, Rzgowska St. 281/289, 93-338 Lodz, Poland
3 Department of General and Oncological Surgery, Medical University of Lodz, Parzeczewska St. 35, 95-100 Zgierz, Poland

*Author to whom correspondence should be addressed.

Academic Editor: Anna Czarnecka

Front. Biosci. (Elite Ed) 2011, 3(1), 137–157; https://doi.org/10.2741/E228
Published: 1 January 2011
(This article belongs to the Special Issue Frontiers in molecular medicine)
Abstract

In the present study the role of tumour suppressor genes (TSGs) hypermethylation and genetic instability of LOH/MSI type in thyroid tumorigenesis was assessed. Expression, methylation status and presence of LOH/MSI were analyzed for 8 TSGs selected from imprinted (IR) and non-imprinted (NIR) chromosomal regions in papillary thyroid carcinomas (PTCs) and nodular goitres (NGs). The results show that methylation-induced gene silencing occurs at an early step of thyroid carcinogenesis and involves multiple genes. Genetic changes of LOH/MSI type are less frequent. In PTC samples, the lack of significant differences in the frequency of LOH in IR and NIR suggests that it is not a key mechanism changing the pattern of gene expression. Co-methylation observed both in NG and PTC raises a possibility that, in thyroid tissue, methylation-induced silencing may occur not only in malignant transformation but also in functional context. We did not recognize any of the studied TSGs – in regard to aberrant methylation status or LOH/MSI frequency – as a selective molecular marker in thyroid tumorigenesis.

Keywords
Papillary thyroid carcinoma
Nodular goitre
Tumour suppressor gene
Imprinted region
Non-imprinted region
Methylation pattern
Methylation index
Methylation-specific PCR
Bisulfite-specific PCR
Realtime PCR
Gene expression
Loss of heterozygosity
Microsatellite instability
Fractional allele loss
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