IMR Press / FBE / Volume 3 / Issue 1 / DOI: 10.2741/E225

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Spore trap analysis and MSQPCR in evaluating mold burden: a flooded gymnasium case study

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1 Mycometrics, LLC, 11 Deer Park Drive, Suite 210, Monmouth Junction, NJ 08852, USA
2 Environmental Analytics, P.O. Box 57605, Tucson, AZ 85732, USA
3 EHS Services, LLC, 115 Hazel Path, Suite 3, Hendersonville, TN 37075, USA
4 United States Environmental Protection Agency (USEPA), 26 West M. L. King Drive, Cincinnati, OH 45268, USA

*Author to whom correspondence should be addressed.

Academic Editor: Atin Adhikari

Front. Biosci. (Elite Ed) 2011, 3(1), 108–114; https://doi.org/10.2741/E225
Published: 1 January 2011
Abstract

A school gymnasium was accidentally flooded by the fire-suppression sprinkler system. The surface water was removed immediately and, after 10 days, a professional firm engaged to dry the environment. Twenty five days after the flooding, the school decided to evaluate whether there was any mold growth in the gymnasium. The inspector used two approaches, traditional air samples or a DNA-based analysis of dust samples. Thirty five-minute air samples (for total of 75 L of air each sample) were collected with Air-O-Cell™ (AOC) cassettes and the mold structures (MS) were quantified by microscopy. These samples were compared to two identical outdoor air samples. As an alternative, two dust samples were collected and quantified by mold specific quantitative PCR (MSQPCR). Comparisons of indoor to outdoor mold concentrations in air samples were inconclusive, but applying MSQPCR to the investigation of this water-damaged environment provided a more reliable and useful answer to the extent of mold contamination than did the spore trap analysis.

Keywords
Mold
ERMI
Water-damage
MSQPCR
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