IMR Press / FBE / Volume 2 / Issue 3 / DOI: 10.2741/E143

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Increased miR-21 expression during human monocyte differentiation into DCs
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1 Department of Immunology, Oslo University Hospital, Faculty Division, The Norwegian Radium Hospital, Oslo, Norway
2 Department of Tumor Biology, Oslo University Hospital, Faculty Division, The Norwegian Radium Hospital, Oslo, Norway
Academic Editor:Mouldy Sioud
Front. Biosci. (Elite Ed) 2010, 2(3), 818–828; https://doi.org/10.2741/E143
Published: 1 June 2010
(This article belongs to the Special Issue RNA interference: from basic to medical applications)
Abstract

Differentiation of monocytes into dendritic cells (DCs) is characterised by marked changes in gene expression. The role of microRNAs (miRNAs), a new class of small endogenous non-coding regulatory RNAs, in this process is still unclear. We identified miR-223, miR-16, miR-191, miR-24, let-7b, and miR-21 as differentially expressed between monocytes and monocyte derived DCs. We evaluated the expression levels of computationally predicted target genes of miR-21 in human monocytes following stimulation with GM-CSF and IL-4. Moreover, transfection of monocytes with synthetic miR-21 inhibited the expression of a set of genes that were also repressed during monocyte differentiation to DCs in response to GM-CSF and IL-4. Among these, we identified genes that are involved in cell cycle, apoptosis and differentiation such as PDE4B, PDCD4, ANXA1, ING3, STAG2, TGFBI, S100A12, LAT2 and NRIP1. Collectively, the present study highlights the involvement of miRNAs, particularly miR-21 in monocyte differentiation to DCs and identifies potential miR-21 target genes.

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