IMR Press / FBE / Volume 2 / Issue 3 / DOI: 10.2741/E140

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Proteomics reveals high levels of vitamin D binding protein in myocardial infarction
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1 Division of Cardiology,“Magna Graecia” University, Catanzaro, Italy
2 Laboratory of Molecular and Cellular Cardiology,“Magna Graecia” University, Catanzaro, Italy
3 Laboratory of Proteomics and Mass Spectrometry,“Magna Graecia” University, Catanzaro, Italy
4 Department of Experimental and Clinical Medicine,“Magna Graecia” University, Catanzaro, Italy
5 Division of Cardiac Surgery, Second University of Naples, Naples, Italy
Academic Editor:Giovanni Li Volti
Front. Biosci. (Elite Ed) 2010, 2(3), 796–804; https://doi.org/10.2741/E140
Published: 1 June 2010
(This article belongs to the Special Issue Biochemical markers in biological fluids)
Abstract

The pathogenic mechanisms underlying the disease processes in cardiovascular disease are likely to involve significant alterations in myocardial gene and protein expression. Proteomics analysis can define new protein and peptide changes associated with myocardial infarction (MI). The aim of the present study was to analyze serum proteome of patients with ST-Elevation MI (STEMI). Serum samples were collected from STEMI patients (age 65.0+/-10.3) at 5.3+/-2.7 hours after the onset of typical chest pain and before initiating standard therapy. Ten age- and sex-matched donors were used as controls. The samples were albumin- and IgG-depleted. Isotope-coded affinity tag method was employed to label cysteine residues and liquid chromatography-Tandem Mass Spectrometry analysis was performed to measure the labelled proteins. Our proteomic approach identified increased levels of vitamin D-binding protein precursor (VDB) in the serum from STEMI patients compared to control donors. Western blot analysis confirmed the increase in VDB protein in STEMI patients. Moreover, fresh thrombotic plaques, obtained during primary angioplasty, showed high expression of VDB protein. Finally, VDB protein reduced the aggregation rate and prolonged coagulation time.

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