IMR Press / FBE / Volume 2 / Issue 2 / DOI: 10.2741/E135

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
AtTFC B is involved in control of cell division
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1 Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, The National Plant Gene Research Center, Peking University, Beijing 100871, China
2 National Center for Plant Gene Research, Beijing 100101, China
3 State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100094, China
4 Yunnan Provincial Key Laboratory of Agri-Biotechnology, Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2010, 2(2), 752–763; https://doi.org/10.2741/E135
Published: 1 January 2010
Abstract

Tubulin-folding cofactors play important roles in regulating plant development. Arabidopsis tubulin-folding cofactor B (AtTFC B) is an Arabidopsis homolog of mammalian tubulin-folding cofactor B, whose biological function in plant development remains poorly understood. Here we report that the homozygous attfc b (-/-) allele caused embryonic lethality. Embryogenesis was arrested at early embryo stage and the cells contained one or multiple nuclei. Plants carrying a heterozygous attfc b (+/-) allele exhibited enlarged mesophyll cells and leaf epidermal cells with bulged nuclei. Flow cytometry analysis showed increased ploidy in the leaves of the attfc b (+/-) mutant, as well as increased levels of Cdc2A and CycB1;1. In addition, immunofluorescence assay showed increased numbers of spindles and phragmoplasts in the attfc b (+/-) mutant. These results suggest that AtTFC B plays an important role in plant cell division.

Keywords
AtTFC B
Cell Division
Embryo
Microtubule
Nucleus
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