IMR Press / FBE / Volume 2 / Issue 2 / DOI: 10.2741/E129

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Colorectal cancer detection by means of optical fluoroscopy. A study on 494 subjects
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1 Medical Physics Unit, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
2 Division of General Surgery B, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy
3 Laboratory of Medicine, Fondazione IRCCS Istituto Nazionale Tumori, Milan, Italy

*Author to whom correspondence should be addressed.

Academic Editor: Giovanni Li Volti

Front. Biosci. (Elite Ed) 2010, 2(2), 694–700; https://doi.org/10.2741/E129
Published: 1 January 2010
(This article belongs to the Special Issue Biochemical markers in biological fluids)
Abstract

In the present work we investigated the possible role of the native fluorescence of blood plasma in the management of colorectal cancer (CRC) and its feasibility as a new tumor marker. Sample of blood was collected from 248 asymptomatic blood donors and from 246 CRC patients. The native fluorescence of blood plasma was measured using a conventional spectrofluorimeter. The intensity of fluorescence of blood plasma at 623nm (IF623), reasonably ascribed to endogenous porphyrins, was significantly higher in CRC patients than in healthy subjects. The diagnostic capability of IF623 in the discrimination between healthy subjects and CRC patients was tested by Receiving Operating Characteristic (ROC) curve analysis, which resulted in an Area Under the Curve (AUC) of 0.72+/-0.01. Fluorescence measurement of blood plasma might be considered diagnostically useful as a candidate for a new tumor marker for CRC management. The procedure is characterised by a great acceptability and by a very low cost, and might be used in a two-step screening wherein an IF623 positive result is followed by colonoscopy.

Keywords
Colorectal Tumors
Carcinoma
Adenocarcinoma
Adenoma
Screening
Fluorescence
Tumor Markers
Porphyrins
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