IMR Press / FBE / Volume 2 / Issue 2 / DOI: 10.2741/E128

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

β-Cyclodextrin in DNA decompaction: An imaging approach

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1 MEMPHYS - Center for Biomembrane Physics, Department of Physics and Chemistry, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark

*Author to whom correspondence should be addressed.

Academic Editor: Paula V. Messina

Front. Biosci. (Elite Ed) 2010, 2(2), 684–693; https://doi.org/10.2741/E128
Published: 1 January 2010
(This article belongs to the Special Issue Bioorganic and biophysical chemistry: from basics to applications)
Abstract

In the present work we investigate the CTAB:DNA complexes decompaction process using β-CD as a decompacting agent. The transition from globules (compacted DNA) to coils (decompacted DNA) was achieved without a coexistence region between coils and globules. This non first-order transition was investigated combining fluorescence microscopy (FM), cryo-transmission electron microscopy (cryo-TEM) and TEM of negatively stained samples. Additionally the presence of multi-globular aggregates in the proximity of the critical transition was elucidated. A possible mechanism for the decompaction process was suggested.

Keywords
DNA
Compaction
Decompaction
Surfactants
Cyclodextrins
cryo-TEM
TEM
negative staining
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