IMR Press / FBE / Volume 2 / Issue 2 / DOI: 10.2741/E115

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
A novel testis-specific Na+/H+ exchanger is involved in sperm motility and fertility
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1 State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2 School of Life Science, SunYat-Sen University, Guangzhou 510275, China
3 National Center for Molecular Genetics and Breeding of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

*Author to whom correspondence should be addressed.

Academic Editors: Yi-Xun Liu, Su-Ren Chen

Front. Biosci. (Elite Ed) 2010, 2(2), 566–581; https://doi.org/10.2741/E115
Published: 1 January 2010
(This article belongs to the Special Issue Advances in mammalian reproduction)
Abstract

Sodium-hydrogen exchanger as a channel for regulation of intracellular pH might be a crucial modulator of sperm capacitation and motility. Three members of this family have been identified in spermatozoa. A novel protein testis-specific sodium-hydrogen exchanger named mtsNHE was cloned in the present study. The mtsNHE localizing on principle piece of sperm flagellum contained 12 predicted transmembrane regions without cytoplasmic fragment at carboxyl terminus. Hydrophilic region was common in the sodium-hydrogen exchanger family members. Polyclonal antibodies to trans-membrane region significantly reduced sperm motility, acrosome reaction and ratio of in vitro fertilization. By in-pouring the antibodies in sperm solution, intracellular pH and calcium concentration were decreased. Muscle injection of female mice with the specific gene vaccine of mtsNHE, significantly stepped down fertility rate. Considering its specific expression and involvement in the regulation of fertility, the mtsNHE might be a potential target molecule for developing a new male contraceptive.

Keywords
Mouse
Sodium-Hydrogen Exchanger
Testis
Sperm Specific Antigen
Sperm Motility
Fertility
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