IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E92

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Ablation of iNOS delays cardiac contractile dysfunction in chronic hypertension
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1 Department of Medicine, Section of Cardiology, Center for Cardiovascular Research, University of Illinois at Chicago, USA
2 Department of Physiology and Biophysics, Center for Cardiovascular Research, University of Illinois at Chicago, USA
3 Department of Physiology and Department of Cell Biology, Federal University of Paraná, Curitiba, Brazil

*Author to whom correspondence should be addressed.

Academic Editor: Rosana Bassani

Front. Biosci. (Elite Ed) 2010, 2(1), 312–324; https://doi.org/10.2741/E92
Published: 1 January 2010
(This article belongs to the Special Issue Calcium and the heart: signaling and regulation)
Abstract

We investigated the role of inducible NOS (iNOS) on cardiac function during the development of left ventricular hypertrophy. Hypertrophy was induced by pressure-overload via short-term (2.5 months) or long-term (6.5 months) aortic banding (AoB) in wild-type (WT) and iNOS knock out (iNOSKO) mice. Cardiac function was then assessed via echocardiography, in situ hemodynamics and papillary muscle force measurements. Quantitative RT-PCR and Western blots were used to measure expression of hypertrophic gene markers and proteins respectively. Our data demonstrate that increased afterload via AoB leads to increased expression of iNOS that is associated with cardiac dysfunction. In pressure-overload induced hypertrophy, iNOSKO delays both the expression of hypertrophic markers and contractile dysfunction without causing significant changes in the level of hypertrophy. Moreover, after long-term AoB, iNOSKO animals exhibited increased basal cardiac function and an improved response to beta-adrenergic stimulation compared to long-term AoB WT animals. In conclusion, our data demonstrate that NO production via iNOS plays an important role in modulating cardiac function after moderate AoB that mimics long-term hypertension in humans.

Keywords
Nitric Oxide
Aortic Banding
BetaAdrenergic Stimulation
Hypertrophy
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