IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E81

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

LM23 is essential for spermatogenesis in Rattus norvegicus

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1 Graduate School of Peking Union Medical College, #5 Dong Dan San Tiao, Beijing, 100005, China
2 Department of Reproductive Endocrinology, National Research Institute for Family Planning, No. 12 Da Hui Si, Hai Dian District, Beijing 100081, P.R. China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2010, 2(1), 187–194; https://doi.org/10.2741/E81
Published: 1 January 2010
Abstract

LM23 is a gene with testis-specific expression in Rattus norvegicus. To reveal the function of LM23 in the testis, we used lentivirus-mediated RNA interference (RNAi) to knock down LM23 expression in a tissue-specific manner in vivo. A lentiviral vector expressing a short hairpin RNA (shRNA) targeting LM23 was microinjected into the efferent ducts of R. norvegicus testes. The expression of LM23 in the treated testes was significantly knocked down compared with controls. These LM23-shRNA testes contained germ cells arrested at the spermatocyte stage, and showed increased apoptosis and disregulation of some meiotic genes. The results demonstrate the validity of the RNAi approach for targeting LM23 and reveal that LM23 expression in the testis is crucial for meiosis during spermatogenesis in R. norvegicus.

Keywords
LM23
RNAi
lentiviral vector
spermatogenesis
Rattus norvegicus
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