IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E77

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Simultaneous determination of mycotoxins in biological fluids by LC-MS/MS
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1 Department of Food Science, University of Naples Federico II, Portici, Naples, Italy
2 Biochemical and Clinical Laboratory, “S. Martino” Hospital, Genoa, Italy
3 IRCCS Associazione Oasi Maria S.S., Institute for Research on Mental Retardation and Brain Aging, Troina, Enna, Italy
4 Neonatal Intensive Care Unit “Cesare Arrigo” Children Hospital, Alessandria, Italy
5 Department of Pediatrics, “G. Gaslini Children's University” Hospital, Genoa
6 Department of Biochemistry, Medical Chemistry and Molecular Biology, University of Catania, Italy

*Author to whom correspondence should be addressed.

Academic Editor: Diego Gazzolo

Front. Biosci. (Elite Ed) 2010, 2(1), 151–158; https://doi.org/10.2741/E77
Published: 1 January 2010
(This article belongs to the Special Issue Clinical and biochemical markers and fetal-neonatal development)
Abstract

We report a single, reliable, non-invasive analytical method for monitoring the fetal level of exposure to different mycotoxins. We assessed by tandem mass spectrometry levels up to 200 nanog/l for ochratoxin A and 500 nanog/l for aflatoxins in sample of serum (n= 71), urine (n= 18) and amniotic fluid (n= 21) of pregnant women. Aflatoxin G1 was present in one sample of serum (3.48 microg/l) and in four samples of urine (ranging from 14.0 to 18.8 microg/l), ochratoxin A was present in one sample of amniotic fluid (4.26 microg/l), whereas aflatoxin B1 (ranging from 0.4-2 microg/l) and B2 (ranging from 0.3-3 microg/l) were contextually present in two samples of urine. The very few contaminated samples did not allow statistical comparison between subjects grouped according to the frequency of consumption of commonly contaminated foods. Data confirm that mycotoxins can occur in fetal-maternal biological fluids. However, the incidence and the level of exposure to the investigated mycotoxins do not appear to pose risk for the mother and the fetus.

Keywords
Aflatoxins
Ochratoxin A
OTA
LCMS/MS
Biological fluids
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