IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E74

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Mouse microRNA-124 regulates the expression of Hes1 in P19 cells

Chong Wang1,2Nan Yao1,2Cai-Ling Lu2Dan Li1,2Xu Ma1,2,*
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1 Graduate School of Peking Union Medical College, Number 5 Dong Dan San Tiao, Beijing 100005, China
2 Department of Genetics, National Research Institute for Family Planning, 12 Da Hui Si, Beijing 100081, China

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2010, 2(1), 127–132; https://doi.org/10.2741/E74
Published: 1 January 2010
Abstract

MicroRNAs (miRNAs) belong to a conserved class of small non-coding RNAs that are typically 18–25 nucleotides long. They are found in both animals and plants. These small RNAs can regulate gene expression at translational level by interacting with their target messenger RNAs, and they play an essential role in the development of plants and animals. To date, more than 200 miRNAs have been identified in mammals; however, their mRNA targets have not yet been identified. In this study, we demonstrate that the expression of Hes-1, which is a basic helix-loop-helix transcriptional repressor, is regulated by miRNA-124 in P19 cells. Reduction in the levels of miR-124 mediated by locked nucleic acids resulted in the accumulation of Hes-1 and hindered the retinoic acid-induced neuronal differentiation of P19 cells. Thus, our results indicate that miR-124 regulates the expression of Hes-1 at the post-transcriptional level and is involved in the retinoic acid-induced neuronal differentiation of P19 cells.

Keywords
P19
Hes-1
MiRNA-124
Neurogenesis
Cell Differentiation
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