IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E69

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Trans-10,cis-12-CLA dysregulate lipid and glucose metabolism and induce hepatic NR4A receptors
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1 Centro de Investigacion Cardiovascular (CSIC-ICCC), Hospital de la Santa Creu i Sant Pau, c/Antoni Mª Claret 167, 08025 Barcelona, Spain
2 Departamento de Bioquimica y Biologia Molecular y Celular, Facultad de Veterinaria, Instituto Aragones de Ciencias de la Salud (Universidad de Zaragoza-Direccion Salud del Gobierno de Aragon), Miguel Servet 177, 50013 Zaragoza, Spain
3 CIBER de Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, Spain
4 Departamento de Patologia Animal, Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, 50013 Zaragoza, Spain
5 Nutrigenomics Research Group, UCD Conway Institute, University College, Dublin, Ireland

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2010, 2(1), 87–97; https://doi.org/10.2741/E69
Published: 1 January 2010
Abstract

Our aim was to assess the effect of two isomers of conjugated linoleic acids (CLA), cis-9,trans-11-CLA (c9,t11-CLA) and trans-10,cis-12-CLA (t10,c12-CLA), on glucose metabolism and hepatic expression of NR4A receptors, key transcription factors regulating gluconeogenesis. ApoE-deficient mice were fed isocaloric, isonitrogenous westernized diets enriched with c9,t11-CLA, t10,c12-CLA or linoleic acid (control diet). Plasma glucose, NEFA, triglyceride and cholesterol concentrations were significantly higher in the t10,c12-CLA group compared with c9,t11-CLA or control group. Plasma insulin concentrations were lowered by c9,t11-CLA compared with either control or t10,c12-CLA group. Hepatic expression of NR4A receptors (Nur77, Nurr1 and NOR-1) was induced by t10,c12-CLA while c9,t11-CLA had not effect. Consistently t10,c12-CLA up-regulated key genes involved in gluconeogenesis including glucose-6-phosphatase, enolase, phosphoenolpyruvate carboxykinase and pyruvate carboxylase. Hepatic expression of NR4A receptors correlated with plasma NEFA, with the expression of their target gene fatty acid transporter (FAT)/CD36 and with the accumulation of fat in the liver. These results suggest that t10,c12-CLA promote dysregulation of lipid and glucose metabolism, at least in part, by an isomer-specific modulation of hepatic expression of NR4A receptors.

Keywords
Dietary Fatty Acids
Hepatic Lipid Homeostasis
Glucose Metabolism
Gluconeogenesis
Conjugated Linoleic Acids
NR4A receptors
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