IMR Press / FBE / Volume 2 / Issue 1 / DOI: 10.2741/E68

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Electronegative LDL induces Fas and modifies gene expression in mononuclear cells
Show Less
1 Institut de Recerca, Hospital de la Santa Creu i Sant Pau
2 Servei de Bioquimica, Hospital de la Santa Creu i Sant Pau
3 Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2010, 2(1), 78–86; https://doi.org/10.2741/E68
Published: 1 January 2010
Abstract

Electronegative LDL (LDL(-)) is a minor modified LDL subfraction that promotes cytokine release by human mononuclear cells. The aim of the current study was to evaluate changes in gene expression induced by LDL(-) versus native LDL in lymphocytes and monocytes. Therefore, mononuclear cells were incubated with these LDL subfractions and their effects on expression in human whole genome were analyzed by gene array. Differential expression of the genes was quantified by real-time RT-PCR. LDL(-) altered the gene expression pattern, particularly of inflammatory genes. LDL(-) down-regulated CD36 and colony-stimulating factor 1 receptor (CSF1R) genes and up-regulated Fas expression and Fas protein on cellular membrane. LDL(-) seemed to promote the alterations in these genes by activation of NF-kB and inhibition of AP1 and PPARG. In conclusion, LDL(-) induced changes in gene expression in monocytes and lymphocytes. Fas up-regulation suggests a proinflammatory action; however, CSF1R and CD36 down-regulation could decrease monocyte differentiation and activation. Therefore, LDL(-) promoted not only inflammatory effects but also counteracting actions in circulating mononuclear cells.

Keywords
LDL(-)
Monocytes
Lymphocytes
Gene Expression Array
real-time RT-PCR
Fas
CD36
CSFR1
Transcription Factors
Share
Back to top