IMR Press / FBE / Volume 11 / Issue 1 / DOI: 10.2741/E852

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Identification of salt-tolerant cowpea genotypes using ISSR markers and proteome analysis
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1 Department of Biochemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
2 Department of Food Science and Nutrition, Ageing and Dementia Research Group, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud, Sultanate of Oman
3 Department of Cropscience, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud, Sultanate of Oman
4 Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
Send correspondence to: Muthuswamy Anusuyadevi, Department of Biochemistry, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India, Tel: 91-431-2407071, Fax: 91-431-2407045, msanushyas2005@gmail.com
Front. Biosci. (Elite Ed) 2019, 11(1), 130–149; https://doi.org/10.2741/E852
Published: 1 June 2019
Abstract

Soil salinity globally affects the productivity of staple food crops. Therefore, an understanding of the molecular mechanisms that lead to salt tolerance induced by antioxidant mechanisms can assist in the development of salt-tolerant crops. To decipher the molecular fingerprint of salt resistance, in this study, six salt-tolerant cowpea genotypes at the seedling stage were assessed for their antioxidant responses, yield, genetic polymorphism and proteomics under salt stress. Leaves and roots showed distinct tissue-specific responses to salinity, and leaves showed a better protection against salt stress-induced oxidative stress than roots. Inter simple sequence repeat (ISSR) fingerprinting allowed molecular discrimination between salt-tolerant cowpea genotypes. Proteome analysis of cowpea leaves under salt stress revealed up-regulation of ATP synthase, vacuolar ATPase, pentatricopeptide repeat protein, flavanone 3-hydroxylase and outer envelope pore protein. Thus, ISSR and proteome analysis allow the identification of salt-tolerant cowpea cultivars.

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