IMR Press / FBE / Volume 11 / Issue 1 / DOI: 10.2741/E847

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article
Characterization of embryonic cells obtained from multifetal reduction
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1 The Egyptian IVF-ET Center 3 Road 161 Hadayek El-Maadi, Cairo 11431, Egypt
2 Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Cairo, Egypt
3 Center of Excellence for Stem Cells and Regenerative Medicine (CESC), Zewail City of Science and Technology, Giza, Egypt
4 Department of Medical Histology and Cell Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
5 Cytogenetic Department, National Egyptian Research Institute, Cairo, Egypt
6 The Urology and Nephrology Center, Mansoura University. Mansoura, Egypt
Send correspondence to: Nagwa El-Badri, Biomedical Sciences Program, Director, Center of Excellence, for Stem cells and Regenerative Medicine (CESC), Zewail City of Science and Technology, Ahmed Zewail Road, October Gardens, 6th of October City, Giza, Egypt, Building: Helmy Institute of Biomedical Sciences, Room number: F010, Egypt, Tel: 20238540401, Fax: 20 238540401, E-mail: nelbadri@zewailcity.edu.eg.
Front. Biosci. (Elite Ed) 2019, 11(1), 79–88; https://doi.org/10.2741/E847
Published: 1 January 2019
Abstract

The multifetal reduction (MFR) procedure is usually reserved for high-order multiple pregnancies, and aspirated tissues are typically discarded. In this study, cells obtained from MFR tissue (termed multifetal reduction embryonic cells (MFR-ECs)), were characterized in vitro by genotypic and phenotypic analyses and tested in vivo by injection under the kidney capsule of nude mice. MFR-ECs were highly proliferative in culture and showed a normal karyotype by microarray CGH. Immunohistochemical analysis at day zero showed positive focal staining for desmin, S-100 protein, synaptophysin and chromogranin. Histology examination showed a mixture of cells from the three germ layers at different stages of differentiation. Markers of these stages included important developmental transcription factors, such as beta three-tubulin (ectoderm), paired box 6 (ectoderm) and alpha-smooth muscle actin (mesoderm). Quantitative polymerase chain reaction (qPCR) showed down-regulation of the mRNAs of cancer-related genes such as TP53. In vivo transplantation in nude mice showed a typical hyaline cartilage plate and no teratoma formation. Thus, MFR-ECs represent a rich, unique source for studying stem cell development, embryogenesis and cell differentiation.

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