IMR Press / FBE / Volume 10 / Issue 3 / DOI: 10.2741/E834

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Valeriana wallichii extract inhibits tert-BOOH induced oxidative damage and cytotoxicity

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1 Defence Institute of Physiology and Allied SciencesLucknow, Defence Research and Development Organization, Road Timarpur, Delhi 110 054, India
2 Defence Bio-Engineering and Electromedical Laboratory, Defence Research and Development Organization, C V Raman Nagar, Bengaluru - 560 093, India

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2018, 10(3), 469–480; https://doi.org/10.2741/E834
Published: 1 June 2018
Abstract

Oxidative stress is well known to be involved in pathophysiology of several disorders. Valeriana wallichii (VW) root extracts is known for its expedient activities but its antioxidant and cytoprotective efficacy need to be explored further. In present study tertiary-butyl hydroperoxide (tert-BOOH) was used to induce oxidative stress in C6 glioma cells. Antioxidant activity of the VW root extracts were evaluated by chemical assays i.e. DPPH, ABTS and FRAP assay. Further effect of VW on tert-BOOH induced oxidative stress and mitochondrial damage was studied. Result of present study revealed that exposure of cells to tert-BOOH resulted in increase in cytotoxicity, reactive oxygen species (ROS) production and decrease in mitochondrial membrane potential, super oxide dismutase, catalase, glutathione peroxidase and glutathione reductase. On the other hand, pretreatment of cells with VW extracts ameliorated these damaging effects. Additionally, HPLC analysis revealed hesperidin as an active ingredient and concentration of heavy metal was found within the maximum permissible limits prescribed by WHO. In conclusion present study revealed the antioxidant and cytoprotective property of VW against oxidative stress.

Keywords
Oxidative stress
tertiary-butyl hydroperoxide
tert-BOOH
Valeriana wallichii
VW
C6 glioma cells
Antioxidant status
Hesperidine
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