IMR Press / FBE / Volume 10 / Issue 2 / DOI: 10.2741/E823

Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.

Open Access Article

Asiatic acid nullified aluminium toxicity in in vitro model of Alzheimer’s disease

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1 Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamil Nadu 608002, India
2 Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman
3 Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman
4 Food and Brain Research Foundation, Chennai, Tamil Nadu 600094, India
5 Dept of Pharmacology, JSS College of Pharmacy, JSS University, SS Nagar, Mysore - 57 00 15, Karnataka, India

*Author to whom correspondence should be addressed.

Front. Biosci. (Elite Ed) 2018, 10(2), 287–299; https://doi.org/10.2741/E823
Published: 1 January 2018
Abstract

Aluminium (Al) is a ubiquitously distributed environmental toxicant that lacks biological functions; however, its accumulation in the brain has been demonstrated to be linked to several neuropathological conditions particularly Alzheimer’s disease (AD). Asiatic acid (AA), a triterpene extracted from Centella asiatica, has been reported to cross the blood brain barrier and also displayed antioxidant and neuroprotective activities. The present study was aimed to explore the neuroprotective effect of AA against aluminium maltolate (Al(mal)3) induced neurotoxicity by assessing cell viability, mitochondrial membrane potential, levels of reactive oxygen species (ROS), DNA damage and apoptosis (Hoechst and dual staining, comet assay; expressions of pro-apoptotic, anti-apoptotic and signaling indices) via AKT/GSK-3β signaling pathway in SH-SY 5Y neuroblastoma cells. Pre-treatment with AA significantly enhanced cell viability, attenuated rotenone-induced ROS, mitochondrial membrane dysfunction and apoptosis regulating AKT/GSK-3β signaling pathway. Downregulation of Al induced neurodegeneration may be one of the approaches to control the impairment of metal ion homeostasis leading to neuronal injury in early development of AD. However, more extensive work in animal model is desirable to confirm its neuroprotective action.

Keywords
Aluminium Maltolate
Asiatic Acid
Oxidative stress
Mitochondrial dysfunction
Apoptosis
AKT/GSK-3β signaling pathway
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