Frontiers in Bioscience-Elite (FBE) is published by IMR Press from Volume 13 Issue 2 (2021). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with Frontiers in Bioscience.
A variety of gene transfer technologies have been devised as tools for functional analysis of genes as well as gene therapy and regenerative medicine. Electroporation enables efficient genetic transfection in vivo into various organs as well as tumors without the aid of any infectious viral vector, and has been applied to preclinical nonviral gene therapy approaches. The efficacies of transfection and expression can be further elevated when the electroporation procedure was used to deliver Epstein-Barr virus (EBV)-based plasmid vectors that have characteristic artificial chromosome-like functions. Electroporation also facilitates delivery of synthetic short interfering RNA (siRNA) into some organs and tumors, potentially providing feasible strategies of RNAi-based molecular targeting therapy against various diseases including malignancies.