IMR Press / EJGO / Volume 42 / Issue 4 / DOI: 10.31083/j.ejgo4204100
Open Access Original Research
Number of colposcopic cervical biopsies and diagnosis of cervical intraepithelial neoplasia: a prospective study
Show Less
1 Maternal-Infantile Department, Complex Operative Unit of Obstetrics and Gynecology, “Alto Tevere” Hospital of Città di Castello, 06012 Perugia, Italy
2 Department of Medical-Sciences, Section of Obstetrics and Gynecology, University of Ferrara, 44121 Ferrara, Italy
Eur. J. Gynaecol. Oncol. 2021, 42(4), 649–654;
Submitted: 1 April 2021 | Revised: 26 May 2021 | Accepted: 1 June 2021 | Published: 15 August 2021
(This article belongs to the Special Issue Update on Cervical Cancer Prevention and Screening)

Objective: To define the relationship between the number of cervical colposcopic biopsies performed on a patient and the diagnosis of each grade of cervical intraepithelial neoplasia (CIN). Methods: Patients who underwent a colposcopy and biopsy between January and June 2018 in an Italian second-level check-point for cervical cancer screening were prospectively enrolled in the study. Cervical punch biopsies were performed on abnormal acetowhite areas that were identified by colposcopy and endocervical sampling was performed if needed. The number of cervical biopsies per patient was recorded along with the following parameters: type of transforming zone, colposcopic grading, Pap smear result, the patient’s age, and endocervical sampling. All parameters were included in multivariable models. The dependent variable was a diagnosis of CIN-0/1, CIN-2, or CIN-3. Results: Independently of other variables, a Pap test result of atypical squamous cells—cannot be excluded H-SIL (ASC-H), atypical glandular cells, not otherwise specified (AGC-NOS), or high grade squamous intraepithelial lesion (H-SIL) is associated with reduced odds of a CIN-0 or CIN-1 diagnosis. More than one cervical biopsy per patient is associated with reduced odds of a CIN-0 or CIN-1 diagnosis whereas three or four biopsies is associated with increased odds of a CIN-2 diagnosis. A Pap test result of HSIL, ASC-H, or AGC-NOS is the only variable that increased the odds of a CIN-3 diagnosis. Discussion: A greater number of cervical biopsies performed on a patient increases the likelihood of diagnosing a CIN-2 but has no effect on the diagnoses of CIN-0/1 or CIN-3.

Cervical biopsy
Cervical intraepithelial neoplasia
Back to top