IMR Press / EJGO / Volume 40 / Issue 2 / DOI: 10.12892/ejgo4529.2019
Open Access Case Report
RAPH1 in cervical cancer proliferation, migration, and invasion: in vitro studies with SiHa and HeLa cells
H.-F. Chen1,†P. Yang1,†G.-Q. Yang1Y. Lin2J.-K. Li1,2,*
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1 Department of Gynecology and Obstetrics, West China Second Hospital, Sichuan University, Chengdu, China
2 Key Laboratory of Birth Defects and Related Disease of Women and Children (Sichuan University), Ministry of Education, Chengdu, China

Contributed equally.

Eur. J. Gynaecol. Oncol. 2019, 40(2), 342–344; https://doi.org/10.12892/ejgo4529.2019
Published: 10 April 2019
Abstract

Background: Ras association and pleckstrin homology domains 1 (RAPH1) may play a critical role in cervical cancer development, prompting us to investigate its potential role(s) in proliferation, migration, and invasion by cervical cancer cells. Materials and Methods: RAPH1 was knocked down in SiHa and HeLa cervical cancer cells using short interfering RNA. Effects of RAPH1 knock-down were assessed on cell proliferation using the CCK8 assay, and on cell migration and invasion using in vitro transwell assays. Results: Efficient RAPH1 knock-down significant reduced the proliferation, migration, and invasion abilities of SiHa and HeLa cells (p < 0.05). Conclusions: RAPH1 may promote tumor cell proliferation, migration, and invasion during cervical cancer development.

Keywords
RAPH1
Cervical cancer
Cell migration
Figures
Figure 1.
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