Background: Ras association and pleckstrin homology domains 1 (RAPH1) may play a critical role in cervical cancer development, prompting us to investigate its potential role(s) in proliferation, migration, and invasion by cervical cancer cells. Materials and Methods: RAPH1 was knocked down in SiHa and HeLa cervical cancer cells using short interfering RNA. Effects of RAPH1 knock-down were assessed on cell proliferation using the CCK8 assay, and on cell migration and invasion using in vitro transwell assays. Results: Efficient RAPH1 knock-down significant reduced the proliferation, migration, and invasion abilities of SiHa and HeLa cells (p < 0.05). Conclusions: RAPH1 may promote tumor cell proliferation, migration, and invasion during cervical cancer development.