IMR Press / EJGO / Volume 39 / Issue 3 / DOI: 10.12892/ejgo3990.2018

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Effect of siRNA against nerve growth factor (NGF) on growth and invasion of ovarian cancer cell
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1 Department of Gynecology, First People's Hospital of Hangzhou, Hangzhou, China
2 Department of Radiation Oncology, Hangzhou Cancer Hospital, Hangzhou, China
Eur. J. Gynaecol. Oncol. 2018, 39(3), 470–475; https://doi.org/10.12892/ejgo3990.2018
Published: 10 June 2018
Abstract

Objectives: To compare the expression of nerve growth factor (NGF), TrkA, p75, and VEGF in normal ovaries and in epithelial ovarian carcinomas. To examine the effects on ovarian cancer cell line (A2780) proliferation, invasion, and apoptosis after NGF down-regulation induced by lentivirus induced RNAi. Materials and Methods: The expression and localization of NGF, TrkA, p75, and VEGF in normal ovarian samples and in ovarian cancer samples were analyzed by RT-PCR and immunohistochemistry. NGF knockdown in A2780 was achieved by shRNA and cell proliferation, invasion, and apoptosis was examined by MTT, transwell assay, and flow cytometry, respectively. Results: Significantly higher levels of NGF, TrkA, and VEGF were observed in ovarian cancers versus normal ovary, while expression level of p75 maintained stable. In A2780 cells, NGF down-regulation significantly reduced the expression levels of TrkA and VEGF, but did not change that of p75. A significant decrease in proliferation and invasion, as well as an increase in apoptosis was also observed in NGF down-regulation cells. Conclusions: The expression of TrkA and VEGF is importantly induced by NGF. Knockdown of NGF promoted apoptosis, thus inhibited cellular proliferation and invasion in A2780 cells. Therefore NGF block may be a potential therapeutic strategy to treat ovarian cancer.
Keywords
NGF
NGF siRNA
TrkA
p57
VEGF
Ovarian cancer
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