Objective: To assess the knowledge and awareness of Jordanian female university students regarding human papillomavirus (HPV) infection and its vaccine. Materials and Methods: A self-administered, anonymous questionnaire was distributed to 450 participants at Jordan University of Science and Technology (JUST) in Jordan. The survey focused on three areas: knowledge and awareness of HPV infection, knowledge of HPV vaccine, and source of information about HPV infection and its vaccine. Results: The survey response rate was 79.8%. Most of the respondents (about 68%) have heard about HPV and knew that the HPV is a virus in the genital area that is sexually transmitted, and 59% of them knew that persistent infection with HPV virus can cause precancerous/cancerous diseases in the genital tract. Only 45% of the respondents have ever heard about HPV vaccine and 37.8% of them knew that HPV vaccine is very effective in preventing HPV infection and its related precancerous/cancerous diseases. Majority of the respondents, 78.7 % and 73.5%, who knew about HPV and HPV vaccine, respectively, had heard about it from healthcare providers. Conclusion: The study suggests that more health education and information about the HPV characteristics and HPV vaccine is needed for female university students.