IMR Press / EJGO / Volume 37 / Issue 1 / DOI: 10.12892/ejgo2765.2016

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Plumbagin shows anti-cancer activity in human breast cancer cells by the upregulation of p53 and p21 and suppression of G1 cell cycle regulators
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1 Department of Oncology, People's Hospital of Jiangxi province, Nanchang University, Nanchang, China
2 Department of Rehabilitation, The First Affiliated Hospital Of Nanchang University, Nanchang, China
3 Department of General Surgery, People's Hospital of Jiangxi province, Nanchang University, Nanchang, China
4 Department of Oncology, The First Affiliated Hospital Of Nanchang University, Nanchang, China
Eur. J. Gynaecol. Oncol. 2016, 37(1), 30–35; https://doi.org/10.12892/ejgo2765.2016
Published: 10 February 2016
Abstract

Purpose: Plumbagin, a naphthoquinone constituent of Plumbago zeylanica L. (Plumbaginaceae), is known to exhibit proapoptotic, antiangiogenic and antimetastatic effects in cancer cells. However, the effect of Plumbagin on breast cancer cells and the underlying molecular mechanism has not yet been elucidated. Materials and Methods: MCF-7 (a human breast cancer cell line) was exposed different concentrations of Plumbagin (PG), and the anti-proliferative activity was evaluated by the MTT assay. The mechanism of action for the growth inhibitory activity of Plumbagin on MCF-7 cancer cells was evaluated using flow cytometry for cell cycle distribution, and western blot for assessment of expression of potential target proteins. Results: Plumbagin exhibited a significant anti-proliferative activity against human breast cancer cells. Flow cytometric analysis revealed that Plumbagin caused cell cycle arrest at G1 phase. The cell cycle arrest was well correlated with the inhibition of cyclin D1, cyclin E, and upregulation of tumor suppressor protein p53. It further inhibited the expression of anti-apoptotic Bcl-2 family members such as Bcl-xL and Bcl-2, and activated pro-apoptotic proteins like Bax and Bak. Conclusion: These findings suggest that the anti-proliferative effect of Plumbagin is due to upregulation of p53 and p21 and suppression of G1 cell cycle regulators.
Keywords
Plumbagin
MCF-7
Cyclin D1
Cyclin E
p53
Proliferation
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