IMR Press / EJGO / Volume 37 / Issue 1 / DOI: 10.12892/ejgo2744.2016

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Effects of silencing MTA1 gene by RNA interference on invasion and metastasis of endometrial carcinoma
C. Su1,*M. Fan2L. Lu3P. Li4
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1 Department of Obstetrics and Gynecology, the Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou City, China
2 Department of Ultrasound, the First Affiliated Hospital of Zhengzhou University, Zhengzhou City, China
3 Department of Radiology, the Third Affiliated Hospital of Zhengzhou University, Zhengzhou City, China
4 Department of Pathphysiology, Zhengzhou University, Zhengzhou City, China
Eur. J. Gynaecol. Oncol. 2016, 37(1), 59–62; https://doi.org/10.12892/ejgo2744.2016
Published: 10 February 2016
Abstract

Objective: The study aimed at the silence effect of MAT1 gene and proteins in Ishikawa cell of human endometrial carcinoma cell line through RNAi mediated by siRNA and also discussed the effect of MTA1 gene silence on the invasion and metastasis of endometrial carcinoma. Materials and Methods: According to the principle of designing siRNA sequence, siRNA aiming at MTA1 gene (MTA1- siRNA) and siRNA used for negative control (Control-siRNA) were designed and synthesized and Ishikawa cell was transfected by transfection reagents. RT-PCR method as well as western blot was used, respectively, to detect the MTA1 mRNA and protein expression of stably transfected cells. Transwell method and scarification experiment were adopted to detect the invasion and metastasis of Ishikawa cells. Results: The expression results of MTA1 on the levels of mRNA and protein showed that the expression level in transfected MTA1-siRNA group was obviously lower than that in non-transfected group and transfected control-siRNA group (p < 0.05), while there was no significant difference between non-transfected group and transfected control-siRNA group (p > 0.05). Effective interference on the expression of MTA1 gene remarkably lowered the invasion and metastasis of endometrial carcinoma Ishikawa cells. Conclusion: RNAi aiming at MTA1 can effectively inhibit the expression of MTA1 in endometrial carcinoma Ishikawa cells and the effective silence of MTA1 can weaken the invasion and metastasis of Ishikawa cells, which provides a new strategy for gene therapy of endometrial carcinoma and an experimental basis for inhibiting the invasion and metastasis of endometrial carcinoma.
Keywords
Endometrial carcinoma
MTA1
RNAi
Invasion and metastasis
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