IMR Press / EJGO / Volume 36 / Issue 5 / DOI: 10.12892/ejgo2730.2015

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Extracellular matrix metalloproteinase inducer (EMMPRIN) remodels the extracellular matrix through enhancing matrix metalloproteinases (MMPs) and inhibiting tissue inhibitors of MMPs expression in HPV-positive cervical cancer cells
Q. Xu1,†X. Cao2,†J. Pan3,*Y. Ye4Y. Xie4N. Ohara5H. Ji1
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1 Department of Gynecology Oncology, Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou
2 Department of Radiation Oncology, Cancer Center, Sun Yat-sen University, Guangzhou
3 Department of Radiation Oncology, Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou
4 Department of Research Center, Fujian Provincial Cancer Hospital, Teaching Hospital of Fujian Medical University, Fuzhou (China)
5 Department of Obstetrics and Gynecology, Sanda Municipal Hospital, Sanda (Japan)
These authors contributed equally.
Eur. J. Gynaecol. Oncol. 2015, 36(5), 539–545; https://doi.org/10.12892/ejgo2730.2015
Published: 10 October 2015
Abstract

Purpose of investigation: To study the expression of extracellular matrix metalloproteinase inducer (EMMPRIN), matrix metalloproteinases (MMPs), and tissue inhibitors of MMP (TIMPs) in uterine cervical cancer cell lines in vitro. Materials and Methods: EMMPRIN, MMPs, and TIMPs expression were assessed by Western blot and real-time RT-PCR from cervical carcinoma SiHa, HeLa, and C33-A cells. Results: EMMPRIN recombinant significantly increased MMP-2, MMP-9 protein and mRNA expression in SiHa and Hela cells, but not in C33-A cells by Western blot analysis and real-time RT-PCR. EMMPRIN recombinant significantly inhibited TIMP-1 protein and mRNA levels in SiHa and Hela cells, but not in C33-A cells. There was no difference on the TIMP-2 expression in those cells with the treatment of EMMPRIN recombinant. EMMPRIN RNAi decreased MMP-2 and MMP-9 and increased TIMP-1 expression in SiHa and HeLa cells, but not in C33-A cells. There was no change on the expression of TIMP-2 mRNA levels in SiHa, HeLa and C33-A cells transfected with siEMMPRIN. Conclusions: EMMPRIN may induce MMP-2 and MMP-9, and downregulate TIMP-1 in HPV-positive cervical cancer cells in vitro.
Keywords
Extracellular matrix metalloproteinase inducer (EMMPRIN)
Matrix metalloproteinases (MMPs)
Tissue inhibitors of MMP (TIMP)
Cervical carcinoma
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