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European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Screening of the miRNAs related to breast cancer and identification of its target genes
Xianfu Sun1, Suxia Luo2, Yaning He1, Yingbo Shao1, Chaojun Liu1, Qi Chen1, Shude Cui1, Hui Liu1,*
1 Department of Breast, Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou
2 Department of Oncology, Henan Cancer Hospital, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou (China)
Eur. J. Gynaecol. Oncol. 2014, 35(6), 696–700; https://doi.org/10.12892/ejgo25212014
Published: 10 December 2014
Objective: To predict and verify the target genes of the miRNAs related to breast cancer beginning from the miRNA expression profile of human breast cancer. Materials and Methods: The total RNA was extracted from cancer tissues and the corresponding paracancerous tissues of eight breast cancer patients, and then miRNAs were separated. Human breast cancer cell line MDA-MB-231 and the normal mammary epithelial cell line HBL-100 were cultured, and the total RNA was extracted, respectively, with separation of miRNAs. The gene chip technology was used to analyze and detect the miRNAs differentially expressed in tissues and cancer cells. The miRNAexpression profile of human breast cancer was obtained through chip scanning and data analysis. Results: Through dual-luciferase method, it was verified that PDCD4 and PDCD10 were real target genes of miR-21 and miR-200c, respectively. Conclusion: miR-21 and miR- 200c are related miRNAs to breast cancer, and they are associated with the occurrence and development of breast cancer.