IMR Press / EJGO / Volume 35 / Issue 4 / DOI: 10.12892/ejgo24912014

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Curcumin induces human SKOV3 cell apoptosis via the activation of Rho-kinase
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1 Department of Obstetrics, Linyi People's Hospital, Linyi (China)
Eur. J. Gynaecol. Oncol. 2014, 35(4), 433–437; https://doi.org/10.12892/ejgo24912014
Published: 10 August 2014
Abstract

Objective: Curcumin has been showed anti-inflammation and anti-cancer effect in various cancer cells such as lung cancer, breast cancer, and so on. However the pro-apoptosis effect and the mechanism of curcumin in ovarian cell is still not very clear. In this study, the authors demonstrated that curcumin induced human SKOV3 cell apoptosis and explored the underlying mechanism concerning Rho A/Rho-kinase pathway. Materials and Methods: Human SKOV3 cell was performed with MTT assay to measure the cell viability with curcumin. The cell was treatment with 15 μM or 30 μM curcumin and flow cytometry. Cell apoptosis analysis was performed to measure the cell apoptosis level. In order to explore the mechanism concerning pro-apoptosis activity of curcumin, the cells were pre-treatment with Y-27632, a specific Rho-kinase inhibitor, before curcumin was added. Then the expression of activated caspase-3 and Rho A, Rho-kinase was detected by western blot. Results: Treatment with 15 μM or 30 μM curcumin significantly promoted the apoptosis of SKOV3 cell (p < 0.05) and the apoptosis rate is dose-dependent. Curcumin also activated the expression of Rho A and Rho-kinase in a dose-dependent effect. When pre-treatment with Y-27632, the expression of activated caspase-3 was significantly decreased compared to the group without Y-27632 pre-treatment (p < 0.05). Conclusions: Curcumin induced human SKOV3 cell apoptosis in a dosedependent effect. The pro-apoptosis effect of curcumin is partly mediated via the activation of Rho A/Rho-kinase signal pathway. This may help to further clarify the mechanisms of curcumin in ovarian cancer therapy.
Keywords
Ovarian cancer
Curcumin
Apoptosis
Rho-kinase
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