IMR Press / EJGO / Volume 33 / Issue 3 / pii/1631086569918-200845430

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Expression of P-Akt, NFκB and their correlation with human papillomavirus infection in cervical carcinoma
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1 Department of Gynecology, The First Affiliated Hospital of He’nan, University of Sience and Technology, Luoyang (China)
Eur. J. Gynaecol. Oncol. 2012, 33(3), 274–277;
Published: 10 June 2012
Abstract

Purpose: To investigate the expression of P-Akt and NFκB and their correlation with human papillomavirus (HPV) infection in cervical carcinoma. Material and Methods: Expression of P-Akt and NFκB was detected by an immunohistochemical SP technique with HPV DNA detetion by PCR in 26 cases of cervical carcinoma tisuues, 18 cases of cervical intraepithelial neoplasia tisuues (CINI / n = 5, CINII / n = 3, CINIII / n = 10) and 19 cases of chronic cervicitis tissues. The different expressions of P-Akt and NFκB were compared in different pathological types of cervical carcinoma (cervical squamous cell carcinoma, cervical adenocarcinoma), different pathological grading (high, medium, poorly differentiated) and different clinical stage (FIGO I to IV). The relationships between P-Akt and NFκB, respectively, with HPV infection in cervical carcinoma were analyzed. Results: The positive expression rate of P-Akt in chronic cervicitis tissues, CIN and cervical carcinoma tisuues was 21.05%, 66.67%, and 92.31%, respectively. There was no obvious difference in the expression of P-Akt in cervical carcinoma in different pathological types or in pathological grading and no obvious difference in different clinical stages. The positive expression rate of NFκB in chronic cervicitis tissues, CIN and cervical carcinoma tissues was 10.52%, 72.22% and 96.15%, respectively; there was no statistically significant difference among the groups for different pathological types and there was no obvious difference in different pathological grading or different clinical stage. There was an obviously positive correlation between P-Akt and NFκB expression rate and degree of disease (r = 0.998, p < 0.05). Cervical carcinoma and CIN cases totaled 44; the positive expression rate of P-Akt was 87.55% in 32 cases of positive HPVDNA of the 44 cases, and the positive expression rate of P-Akt was only 16.70% in 12 cases of negative HPV-DNA of the 44 cases. The positive expression rate of NFκB was obviously higher in the HPV DNA positive than in the HPV-DNA negative cases. There was a statistically significant difference among the groups (p < 0.05). Conclusion: The positive expression rate of P-Akt and NFκB was closely related with cervical disease extent, and closely related with HPV infection in cervical carcinoma. This study suggests that P-Akt and NFκB more probably play an important role in the occurrence of cervical carcinoma.
Keywords
Cervical carcinoma
P-Akt
NFκB
Human papilloma virus
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