IMR Press / EJGO / Volume 31 / Issue 1 / pii/1630984337235-2050274471

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Analysis of the cytogenetic response in peripheral blood lymphocytes from breast cancer patients following chemotherapy
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1 Department of Biological Sciences, Federal University of Triângulo Mineiro, Uberaba, MG
20023 Integrated College Aparício Carvalho-FIMCA, Porto Velho, RO (Brazil)
Eur. J. Gynaecol. Oncol. 2010, 31(1), 75–79;
Published: 10 February 2010
Abstract

The presence of chromosomal aberrations induced in circulating lymphocytes from breast cancer patients during chemotherapy was analyzed. Ten breast cancer patients undergoing neoadjuvant chemotherapy and ten healthy women (controls) were evaluated. Metaphases were obtained from cultures of peripheral lymphocytes stimulated with phytohemaglutinin and metaphase blockage was achieved with colchicine. One hundred metaphases were analyzed for chromosomal aberrations and 1,000 cells for the mitotic index. No significant differences were observed regarding the frequency of chromosomal aberrations, number of cells with chromosomal aberrations and mitotic index between the controls and patients before chemotherapy. However, after the first chemotherapy cycle, the numbers of chromosomal aberrations and cells with them was greater. After the third cycle, the mitotic index was lower, but the fifth cycle produced an increase in relation to the third and fourth cycles. The results suggest that chemotherapy raises the number of chromosomal aberrations and favors persistence of stable chromosomal abnormalities.
Keywords
Breast cancer
Chemotherapy
Chromosomal changes
Mitotic index
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