IMR Press / EJGO / Volume 30 / Issue 1 / pii/2009013

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Effects of quercetin on proliferation, apoptosis, adhesion and migration, and invasion of HeLa cells

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1 Department of Obstetrics and Gynecology, Renmin Hospital, Wuhan University, Hubei (China)
Eur. J. Gynaecol. Oncol. 2009, 30(1), 60–64;
Published: 10 February 2009
Abstract

Objective: This study sought to explore the effects of quercetin on proliferation, apoptosis, adhesion and migration and invasion of HeLa cells. Methods: Proliferation and apoptosis of quercetin treated HeLa cells were measured by MTT and Annexin V-FITC/PI; adhesion and migration and invasion of quercetin treated HeLa cells were measured by adhesion assay and wound healing assay and the Transwell chamber method, respectively. Results: The data showed that quercetin has functions of inhibiting breeding of HeLa cells and inducing apoptosis of the cells. The total apoptosis rate and cell inhibition rate were positively proportional to concentration of quercetin used. The study suggests that quercetin has functions of inhibiting adhesion and migration and invasion of HeLa cells. The adhesive ratio, migration rate and invasiveness were negatively proportional to concentration of quercetin. The results demonstrated that with quercetin concentration increased from 20 to 80 micromol/l, cell inhibition rate increased from 37% to 83%; cell apoptosis rate increased from 18.71 +/- 2.61% to 70.00 +/- 4.05%; adhesive ratio decreased from 82% to 48%; migration rate decreased from 7.26 +/- 0.20 microm/hr to 3.78 +/- 0.64 microm/hr; invasiveness decreased from 124.33 +/- 1.53 piece to 90.67 +/- 2.08 piece (p < 0. 05). Conclusion: Quercetin can inhibit breeding of HeLa cells and induce apoptosis of the cells. Quercetin can inhibit adhesion and migration and invasion of HeLa cells. Quercetin may play an antimetastatic role.

Keywords
Quercetin
HeLa cell
Cell breeding
Cell apoptosis
Cell adhesion
Cell migration and invasion
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