IMR Press / EJGO / Volume 29 / Issue 3 / pii/1630995590079-11645731

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Cl-channel blockers inhibit cell proliferation and arrest the cell cycle of human ovarian cancer cells
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1 Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan (China)
Eur. J. Gynaecol. Oncol. 2008, 29(3), 267–271;
Published: 10 June 2008
Abstract

Objective: To investigate the role of chloride channels in cell proliferation and cell cycles of human ovarian cancer cell line A2780. Methods: Chloride channel blockers were used to observe the effects of chloride channels on A2780 cells with MTT assay and flow cytometry. Results: NPPB (100 μM) significantly inhibited the cell proliferation and affected the cell cycle, which increased the percentage of cells in the G1 phase, and reduced it in the S phase. NFA (100 μM) and TAM (30 μM) had similar inhibitory effects. Glibenclamide (100 μM), however, had no effect on cell proliferation or cycle. Moreover, chloride channel blockers could inhibit Ca2+ influx in these cells. Conclusion: Chloride channels, voltage-gated chloride channels, and volume-sensitive chloride channels especially, play an important role in the cell proliferation and cycle of A2780 cells. It is likely that the influence of chloride channels on cell proliferation and cell cycle is mediated by a Ca2+-dependent mechanism.
Keywords
Chloride channels
Cell proliferation
Cell cycle
Ovarian cancer
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