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Cl-channel blockers inhibit cell proliferation and arrest the cell cycle of human ovarian cancer cells
1 Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan (China)
Eur. J. Gynaecol. Oncol. 2008, 29(3), 267–271;
Published: 10 June 2008
Objective: To investigate the role of chloride channels in cell proliferation and cell cycles of human ovarian cancer cell line A2780. Methods: Chloride channel blockers were used to observe the effects of chloride channels on A2780 cells with MTT assay and flow cytometry. Results: NPPB (100 μM) significantly inhibited the cell proliferation and affected the cell cycle, which increased the percentage of cells in the G1 phase, and reduced it in the S phase. NFA (100 μM) and TAM (30 μM) had similar inhibitory effects. Glibenclamide (100 μM), however, had no effect on cell proliferation or cycle. Moreover, chloride channel blockers could inhibit Ca2+ influx in these cells. Conclusion: Chloride channels, voltage-gated chloride channels, and volume-sensitive chloride channels especially, play an important role in the cell proliferation and cycle of A2780 cells. It is likely that the influence of chloride channels on cell proliferation and cell cycle is mediated by a Ca2+-dependent mechanism.