IMR Press / EJGO / Volume 27 / Issue 5 / pii/2006216

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Telomerase activity and the subunit of telomerase in hydatidiform mole and their relationship with the development of postmolar tumor

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1 Department of Obstetrics and Gynaecology, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China
2 Department of General Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, China
Eur. J. Gynaecol. Oncol. 2006, 27(5), 473–476;
Published: 10 October 2006
Abstract

Objectives: To investigate the pattern of telomerase activity and the subunit of telomerase in normal placentae and GTD, and to determine the prognostic significance of telomerase activity and the subunit of telomerase in GTD. Methods: Telomerase activity human telomerase (hTERT) and human telomerase (hTR) expression were analyzed in the initial uterine evacuation specimen of 63 hydatidiform moles (HMs), 42 normal human placental tissues, 17 malignant gestational tro­phoblastic tumors, primary cultures of normal villi and JAR cell lines by use of the polymerase chain reaction-based telomeric repeat amplification protocol (TRAP) assay and reverse transcription-polymerase chain reaction (RT-PCR) methods. Results: Telomerase activity was 100% in primary cultures of normal villi and JAR cell lines and in less than 60-day early pla­cental villi, while only 9.1% in greater than 60-day placental villi, 27% in HMs and 58% in malignant trophoblastic tumors. High levels of hTR could be found in all groups. hTR expression was detected in all cases of <60-day placental villi, in 72.7% >60-day placental villi, in 87.3% in HMs and 100% in malignant trophoblastic tumors. Telomerase activity and hTERT expression had sig­nificant differences among the groups. Telomerase activity was associated with serum hCG levels but not related to other clinical risk factors. Conclusions: Telomerase activity may be correlated with the development of trophoblastic tumors, and hTERT may be a useful diagnostic marker for detecting the existence of malignant trophoblastic cells.

Keywords
Telomerase activity
hTERT
hTR
Gestational trophoblastic disease
Hydatidiform mole
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