IMR Press / EJGO / Volume 26 / Issue 6 / pii/2005250

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Assessment of human papilloma viral load of archival cervical intraepithelial neoplasia by real-time polymerase chain reaction in a Turkish population

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1 Department of Obstetrics and Gynecology, Faculty of Medicine, Gazi University, Ankara (Turkey)
2 Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara (Turkey)
3 Department of Medical Pathology, Faculty of Medicine, Gazi University, Ankara (Turkey)
Eur. J. Gynaecol. Oncol. 2005, 26(6), 632–635;
Published: 10 December 2005
Abstract

The propose of this study was to determine the existence, and viral load of human papilloma virus (HPV) subtypes 16 and 18 in paraffinized cervical intraepithelial neoplasia (CIN) samples by real-time polymerase chain reaction (RT-PCR). Overall 94 women were included. Of these patients 47 (50%) had CIN I, 27 (28.8%) had CIN II, and 20 (21.2%) had CIN III. HPV positivity for these three groups were 4.2%, 14.8% and 45%, respectively. HPV positivity in CIN III patients was significantly higher than CIN I (OR = 18.41, 95% CI 3.00-145.73; p < 0.001), and CIN II patients (OR= 4.70, 95% CI 1.00-23.76; p = 0.05). The difference between CIN I and II was not significant (p = 0. 18). Viral loads were 102, and 104 copy/ml for two CIN I patients; 102, 103, and 105 for three CIN II patients; and 102, 103, 104, 104,105, 105and 106 copy/ml for eight patients with CIN III. Viral load of the remaining one patient could not be assessed. No significant variance was noted among the groups with respect to viral load (p = 0.73). RT-PCR had important advantages of detecting, typing, and quantifying at the same time. Although HPV positivity was increased signifi­cantly by the degree of lesions, this relation was not observed for viral load.

Keywords
Human papilloma virus
Real-time polymerase chain reaction
Viral load
Cervical intraepithelial neoplasia
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