IMR Press / EJGO / Volume 26 / Issue 1 / pii/2005101

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Distinguished Expert Series

Immunohistochemistry in assessment of molecular pathogenesis of cervical carcinogenesis

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1 Unit of Cytopathology, National Center of Epidemiology, Surveillance and Promotion of Health, National Institute of Health (ISS), Rome (Italy)
Eur. J. Gynaecol. Oncol. 2005, 26(1), 5–19;
Published: 10 February 2005
Abstract

Concerning the prediction of HPV-associated cervical disease, several importance issues are related both to the manage­ment of women with diagnosed CIN and those with cervical cancer. Oncogenic HPV s are capable of contributing to the deve­lopment of malignant phenotype by several different mechanisms, most of which seem to be closely interrelated. Because of the fact that these molecular interactions are mediated by proteins, the logical strategy to dissect the complex molecular pathways is to study the functions of these proteins, utilising the capabilities of immunohistochemistry (IHC). IHC offers practically unlimited possibilities to study any target molecules, against which a monoclonal or polyclonal anti­body can be raised. This review describes the IHC-based strategies used by this author to assess the molecular pathogenesis of cervical cancer and its precursors in a number of large-scale prospective cohort studies conducted during the past 25 years. In the ongoing HPV-PathogenISS study, 13 different markers are being tested to evaluate their predictive value in distinct viral events, e.g. persistence or clearance of high-risk HPV in women treated for CIN. Apart from getting new insights into the molecular pathogenesis of HPV-.associated cervical carcinogenesis, we anticipate the disclosure of individual markers, a set of markers, or an expression profile of any such marker sets that would be of clinical value as predictors of disease outcome in cervical carcinogenesis.

Keywords
Human papillomavirus
Immunohistochemistry
Cervical cancer
CIN
Biomarker
Prognosis
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