IMR Press / EJGO / Volume 25 / Issue 6 / pii/2004271

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Expression of the cell-cycle regulatory proteins (cyclins D1 and E) in endometrial carcinomas: correlations with hormone receptor status, proliferating indices, tumor suppressor gene products (p53, pRb ), and clinicopathological parameters

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1 Department of Forensic Pathology, Medical School, University of Ioannina, Ioannina (Greece)
2 Department of Pathology, Medical School, University of Ioannina, Ioannina (Greece)
3 Department of Pathology, General Hospital “G. Hatzikosta of Ioannina, Ioannina (Greece)
Eur. J. Gynaecol. Oncol. 2004, 25(6), 719–724;
Published: 10 December 2004
Abstract

Purpose of investigation: This study aimed to investigate the immunohistochemical expression of cyclins Dl and E in normal, hyperplastic and neoplastic endometrium, and their correlation with proliferative activity and clinicopathological features. Methods: We carried out immunohistochemical techniques on archived material of formalin-fixed paraffin-embedded tissues using the antibodies against the cyclins Dl and E, PR-ER, p53, Ki67 (MIBl) and pRb with the streptavidin-biotin-peroxidase method in a total of 20 cases of normal endometrium, 32 cases of hyperplastic endometrium and 66 cases of endometrial carcinomas. Results: Cyclin DI and E immunoreactivity was observed in the nuclei of tumour cells in 18.2% and 39.1 %, respectively, of the cases of endometrial carcinomas. Cyclin DI labelling index was not significantly correlated with any of the clinicopathologic para­meters examined. However, there was a significant correlation between the cyclin E labelling index and histological grade of car­cinoma (p = 0.00096), which increased significantly with histological grades of malignancy. We also detected a significant correla­tion between cyclin E and PCNA (p < 0.0001) as well as with the tumor suppressor genes p53 and pRb (p = 0.052 and 0.0002, respectively) in endometrioid endometrial carcinoma. Conclusion: Our results indicate that cyclin E overexpression may be involved in the development and/or proliferation and dif­ferentiation of human endometrioid endometrial carcinoma. lmmunoexpression of cyclin D1 does not appear to be associated with cell-cycle progression in the benign or malignant endometrium.

Keywords
Cyclin DI
Cyclin E
p53
pRb
Endometrial carcinoma
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