IMR Press / EJGO / Volume 25 / Issue 1 / pii/2004102

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Evaluation of DNA methylation in the human genome: Why examine it and what method to use

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1 Division of Gynecologic Oncology, Department of Obstetrics and Gynecology, University of Iowa Hospitals and Clinics, Iowa City, Iowa (USA)
2 Division of Gynecologic Oncology, St. Vincent Hospitals, Indianapolis, Indiana (USA)
Eur. J. Gynaecol. Oncol. 2004, 25(1), 19–24;
Published: 10 February 2004
Abstract

Purpose: Since its discovery 50 years ago, DNA methylation has been found to be an important part of gene regulation. Newer methods of analysis over the last decade have helped further the understanding of this epigenetic phenomenon. The purpose of this article is to describe current methods of analysis and discuss advantages and disadvantages of each and their possible roles in gynecologic malignancies. Results: The methods for analysis of DNA methylation are divided into two major categories: 1) methods which utilize chemical methods or restriction enzymes to differentially cleave at cytosine versus 5-methylcytosine sites, 2) methods which utilize sodium bisulfite (NaHSO3) to specifically convert unmethylated cytosines to uracil (thymine after PCR). This recen­tly developed method appears to be more sensitive and allows the investigator to specifically delineate the study site(s). Conclusion: DNA methylation is important in the human genome. Its role in tumorigenesis is just beginning to be under­stood. While relying upon newly designed methods of analysis, further understanding of this epigenetic phenomenon and its role in gene expression and tumorigenesis will be forthcoming.

Keywords
Methylation
Tumor suppressor genes
CpG
Mutation
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