European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Simultaneous immunohistochemical localization of β-catenin and cyclin D 1 in differentiated but not in undifferentiated human endometrial carcinoma
Purpose: β-catenin plays dual important roles in epithelial cell-cell adhesion in cytoplasm as well as in the nuclear T-cell factor (TCF)/lymphoid enhancing factor-1 (LEF-1) signaling pathway. Abnormal nuclear accumulation of β-catenin promotes colorectal carcinogenesis by triggering the expression of cyclin D 1 gene through the TCF/LEF-1 pathway. The purpose of this study was to investigate the possible involvement of the TCF/LEF-1 pathway in endometrial carcinogenesis. Methods: lmrnunohistochemical localization of β-catenin and cyclin D1 in normal endometrium, hyperplastic endometrium and endometrial carcinoma were assessed on serial tissue sections. Results: Nuclear accumulation of β-catenin was observed in endometrial carcinomas compared with normal endometria. Cyclin DI-positive endometrial cancer cases were β-catenin-positive in the nuclei, especially in 70% (7/10) of Gl and 55.6% (5/9) of G2 differentiated endometrial carcinomas, but never in G3 undifferentiated ones. Conclusions: These results imply that the simultaneous nuclear accumulation of β-catenin and cyclin D1 - suggesting the activation of the TCF/LEF-1 pathway - may be a potential marker for the progression of TypeⅠendometrial carcinogenesis.