IMR Press / EJGO / Volume 22 / Issue 6 / pii/2001201

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research

Glutathione S-transferases P1-1 and A1-1 in ovarian cyst fluids

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1 Departments of Gynaecology and Obstetrics, University Medical Centre, Nijmegen, The Netherlands
2 Departments of Gastroenterology, University Medical Centre, Nijmegen, The Netherlands
Eur. J. Gynaecol. Oncol. 2001, 22(6), 427–432;
Published: 10 December 2001
Abstract

Purpose: The purpose of the present study was to determine the gluthathione S-transferases (GST) Pl -I and A 1-1 levels in cyst fluid from malignant, borderline, and benign ovarian tumors. The clinical relevance of these enzymes in cyst fluid was investigated, including the possible relation with resistance to chemotherapy. Methods: A total of 90 ovarian cysts were punctured for cyst fluid collection. GSTPI-1 and GSTAl-1 concentrations were deter­mined by ELIS A in cyst fluid from 23 malignant, 9 borderline, and 51 benign primary ovarian tumors, and levels were correlated with histopathological data. Results: Significantly higher GSTPl-1 concentrations were found in cyst fluid from malignant (median: 477 ng/mL), compared with benign (median: 52 ng/mL) ovarian cysts(p < 0.0001), as well as in fluid from borderline (median: 366 ng/mL) compared with benign cysts(p < 0.000 I). No significant differences were found in cyst fluid GSTA 1-1 concentrations between the histologic sub­groups. In cyst fluid from malignant tumors higher GSTPI-1 and lower GSTAl-1 concentrations were found in patients with worse prognostic factors: FIGO II-III-IV, grade 2-3, residual tumor> 2 cm, presence of ascites, patients with recurrent disease, and survival, but differences were not significant. In the subgroup of patients that received cisplatin-based chemotherapy (n = 14) signifi­cantly higher GSTPl-1(p = 0.01) concentrations were found in patients with recurrence compared with patients without recurrence. Considering only FIGO stage I patients, a differentiation could be made between patients with or without recurrence based on cyst fluid GSTPl-1 concentrations. Conclusions: Determination of glutathione S-transferases P 1-1 in cyst fluid samples from ovarian tumors can be of additional value in the differentiation between histologic subgroups. In case of possible low malignant potential cysts where sampling of the most representative tissue can be an issue, determination of GSTP-1 concentrations in cyst fluid may optimise histopathologic classification. Cyst fluid GSTP 1-1 seems to be a good marker for aggressiveness of the ovarian tumor, and it may predict response to chemotherapy.

Keywords
Ovarian tumor
Cyst fluid
Glutathione S-transferase
Chemotherapy
Drug resistance
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